Determining IBUs

This image shows how we extract iso-alpha acids from beer in order to find out how many IBUs are present. We acidify the beer sample in order to make the iso-alpha acids (IAAs) less soluble in beer and more soluble in the non-miscible iso-octane. The iso-octane will extract the IAAs and we will use our UV-Vis spectrophotometer to measure the absorbency at 240 nm in order to find out how many IBUs are present in beer.

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